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KMID : 0604520090350010019
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Journal of the Society of Cosmetic Scientists of Korea
2009 Volume.35 No. 1 p.19 ~ p.25
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Screening System Establishment for Potential Anti-wrinkle Agents Using Human Fibroblast Elastase
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Kim Dong-Seok
Oh Mi-Hee
Lee Ju-Eun
Kim Su-Yeon
Kim So-Young
Park Kyoung-Chan
Yun Hye-Young
Baek Kwang-Jin
Kwon Nyoun-Soo
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Abstract
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It has been reported that not only collagen but also elastin contribute to inhibit skin wrinkle formation. Ultraviolet (UV) radiation induces photo-aging on human skin. Because UV radiation increases elastase activity, it is thought that increased elastase activity could be the major reason for skin elasticity reduction and wrinkle formation by UV. In the present study to standardize elastase activity measuring system, purified elastases from porcine pancrease and human neutrophil, and cell extracts of normal human primary fibroblasts, 3T3 mouse fibroblasts, and CCD-25Sk human fibroblasts were used as various enzyme sources. Furthermore, elastase activities were compared according to concentrations of enzyme and substrate and incubation time. Phosphoramidon was used as a positive control to inhibit elastase activities of normal human primary fibroblasts and CCD-25Sk fibroblasts. However, it had no influence on the activity of porcine pancreatic elastase. Therefore, it is suggested that elastase used for testing anti-wrinkle agents should be selected carefully.
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KEYWORD
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human fibroblast, elastase, elastin, aging, wrinkle
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